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IL-10/B-TCGF/CSIF/TGIF, Mouse, Recombinant-1

英文名称：IL-10/B-TCGF/CSIF/TGIF, Mouse, Recombinant

货号：GPCK248

价格： ￥11880.00 ￥3480.00 ￥1180.00 ￥18880.00

规格：
500μg 50μg 10μg 1mg
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产品信息

产品规格 500μg / 50μg / 10μg / 1mg
表达宿主 E.coli
序列信息 MSRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS with polyhistidine tag at the C-terminus.
检索号 P18893.2
分子量 19.69 kDa
表观分子量 19 kDa
标签 His-tag at the C-terminus
生物活性 Measure by its ability to induce MC/9-2 cells proliferation. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant mouse IL-10 is > 1 × 10^6 IU/mg.
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signalling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively.
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