产品详情
产品信息
产品规格 | 10μg |
表达宿主 | E.coli |
序列信息 | MAVRPLRRRQPKKSNELPQANRLPGIFDDVHGSHGRQVCRRHELYVSFQDLGWLDWVIAPQGYSAYYCEGECSFPLDSCMNATNHAILQSLVHLMKPNAVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH with polyhistidine tag at the C-terminus. |
检索号 | AAP74559 |
分子量 | 16.61 kDa |
表观分子量 | 17 kDa |
标签 | His-tag at the C-terminus |
生物活性 | Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 10-19.4 ng/mL. |
纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
内毒素 | < 0.1 EU/μg |
制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
运输条件 | Ambient temperature or ice pack. |
有效期 | 12个月 |
背景介绍 | Bone morphogenetic protein 8A (BMP8A) is a polypeptide member of the TGFβ superfamily of proteins. Like other BMPs, BMP8A is involved in the development of bone and cartilage. BMP8A may be involved in epithelial osteogenesis. It also plays a role in bone homeostasis. Human BMP?8a is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RTPR) between aa residues 263 and 264 to release a 139 aa carboxy?terminal domain. |
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