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Noggin/NOG/SYM1/SYNS1, Human, Recombinant-1

英文名称：Noggin/NOG/SYM1/SYNS1, Human, Recombinant

货号：GPCK365

价格： ￥1680.00 ￥680.00

规格：
50μg 10μg
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产品信息

产品规格 50μg / 10μg
表达宿主 E.coli
序列信息 MQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC with polyhistidine tag at the C-terminus.
检索号 Q13253.1
分子量 24 kDa
表观分子量 27 kDa
标签 His-tag at the C-terminus
生物活性 Measure by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 0.05 μg/mL in the presence of 50 ng/mL of recombinant human BMP-4.
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 Noggin is a bioactive protein intended for use in cell culture applications. Noggin protein which binds to ligands of the TGF-β family and regulates their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures.
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