英文名称：TNF-α/TNFA/TNFSF2 (N-6His), Human, Recombinant
货号：GPCK052
价格： ￥12880.00 ￥3480.00 ￥1180.00 ￥18880.00
产品详情
产品信息
| 产品规格 | 500μg / 50μg / 10μg / 1mg |
| 表达宿主 | E.coli |
| 序列信息 | Gly57-Leu233 |
| 检索号 | P01375 |
| 分子量 | 21.8 kDa |
| 表观分子量 | 18 kDa |
| 标签 | N-6His |
| 生物活性 | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 30-150 pg/mL. |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 0.1 EU/μg |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 100 mM NaCl, pH 7.2. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 背景介绍 | Tumor Necrosis Factor-α (TNF-α) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-α while cells that express CD8 secrete little or no TNF-α. Synthesis of TNF-α can be induced by many different stimuli including interferons, IL2, and GM-CSF. The clinical use of the potent anti-tumor activity of TNF-α has been limited by the proinflammatory side effects such as fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-α mutants with low systemic toxicity has been of intense pharmacological interest. Human TNF-α that binds to murine TNF-R55 but not murine TNF-R7, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-α, which binds to both murine TNF Receptors. Based on these results, many TNF-α mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo. Recombinant Human TNF-α High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8, Lys9, Arg10 and Phe157. This mutant form has been shown to have increased activity with less inflammatory side effects in vivo. |
相关文献
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期刊：NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
DOI：10.1007/s00210-025-03943-0
影响因子：3.1
相关产品： TNF-α/TNFA/TNFSF2 (N-6His), Human, Recombinant, Mouse Tracheal Smooth Muscle Cells
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期刊：LAB ON A CHIP
影响因子：6.1
相关产品： Human Umbilical Vein Endothelial cells, TNF-α/ TNFA/ TNFSF2, Human, Recombinant, Calcein-AM/PI Double Staining Kit
