产品详情
产品信息
产品形态 | 粉末 |
产品规格 | 10μg |
表达宿主 | E.coli |
序列信息 | CDLPQTHNLRNKRALTLLVQMRRLSPLSCLKDRKDFGFPQEKVDAQQIKKAQAIPVLSELTQQILNIFTSKDSSAAWNTTLLDSFCNDLHQQLNDLQGCLMQQVGVQEFPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSANVLGRLREEK with polyhistidine tag at the N-terminus. |
检索号 | P01572.2 |
分子量 | 19.93 kDa |
表观分子量 | 17-25 kDa |
标签 | His-tag at the N-terminus |
生物活性 | Measure by its ability to protect L929 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is < 10 pg/mL. The specific activity of recombinant mouse IFN alpha 1a is > 4 × 10^7 IU/mg. |
纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
内毒素 | < 0.1 EU/μg |
制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
运输条件 | Ambient temperature or ice pack. |
有效期 | 12个月 |
背景介绍 | Interferon alpha-1 or interferon alpha-D, encoded by the human IFNA1gene, is a member of the type I interferon family . It is expressed in peripheral blood leukocytes and lymphoblastoid cells. There are four highly conserved cysteine residues which form two disulfide bonds. One bond is a necessity for biological activity, which plays an critical role in conducting non-specific resistance against a variety of viral infections, and regulation of cell proliferation and modulates immune responses. |
产品推荐
相关推荐
• EGFR/ErbB1/HER1 (C-6His), Human, Recombinant
• IL-10RB (C-Fc), Human, Recombinant
• IL-1R-2/CD121b (C-6His), Mouse, Recombinant
• TNF-R2/CD120b/TNFRSF1B (C-mFc), Human, Recombinant
• CXCL12/SDF-1, Mouse, Recombinant
• IL-18/IL-1F4 (C-6His), Human, Recombinant
• BMP-7, Human, Recombinant
• MCP-1/CCL2 (C-6His), Mouse, Recombinant
• β-NGF/NGF/NGFB (Glu19-Ala241), Human, Recombinant
• VEGF-D/FIGF (C-6His), Mouse, Recombinant
×