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IFNα 1a/Ifa/Ifa8, Mouse, Recombinant-1

英文名称:IFNα 1a/Ifa/Ifa8, Mouse, Recombinant

货号:GPCK310

价格: ¥680.00

规格:
10μg
产品详情

产品信息

产品形态 粉末
产品规格 10μg
表达宿主 E.coli
序列信息 CDLPQTHNLRNKRALTLLVQMRRLSPLSCLKDRKDFGFPQEKVDAQQIKKAQAIPVLSELTQQILNIFTSKDSSAAWNTTLLDSFCNDLHQQLNDLQGCLMQQVGVQEFPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSANVLGRLREEK with polyhistidine tag at the N-terminus.
检索号 P01572.2
分子量 19.93 kDa
表观分子量 17-25 kDa
标签 His-tag at the N-terminus
生物活性 Measure by its ability to protect L929 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is < 10 pg/mL. The specific activity of recombinant mouse IFN alpha 1a is > 4 × 10^7 IU/mg.
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 Interferon alpha-1 or interferon alpha-D, encoded by the human IFNA1gene, is a member of the type I interferon family . It is expressed in peripheral blood leukocytes and lymphoblastoid cells. There are four highly conserved cysteine residues which form two disulfide bonds. One bond is a necessity for biological activity, which plays an critical role in conducting non-specific resistance against a variety of viral infections, and regulation of cell proliferation and modulates immune responses.
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