请输入关键词！

文献(15) 说明书
一键复制产品信息
EGF/Urogastrone/URG, Human, Recombinant-1

英文名称：EGF/Urogastrone/URG, Human, Recombinant

货号：GPCK001

价格： ￥880.00 ￥680.00 ￥380.00 ￥180.00 ￥1080.00

规格：
500μg 100μg 50μg 10μg 1mg
产品详情

产品信息

产品规格 500μg / 100μg / 50μg / 10μg / 1mg
表达宿主 E.coli
序列信息 MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus.
检索号 P01133.2
分子量 7.16 kDa
表观分子量 9-11 kDa
标签 His-tag at the C-terminus
生物活性 Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately > 1.4 × 10^6 IU/mg.
纯度 > 95% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 Epidermal growth factor (EGF) stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is a 6-kDa protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
相关文献
产品推荐
-产品推荐-9
-产品推荐-9
-产品推荐-9
-产品推荐-9
-产品推荐-9
-产品推荐-9
联系我们
sales@procell.com.cn
400-999-2100
相关推荐
×
确定