英文名称：FGF-2/bFGF/FGF-b (Q65I, C96S, N111G), Human, Recombinant
货号：GPCK006
价格： ￥5080.00 ￥1180.00 ￥580.00 ￥7480.00
产品详情
产品信息
| 产品规格 | 500μg / 50μg / 10μg / 1mg |
| 表达宿主 | E.coli |
| 序列信息 | Met1-Ser155 |
| 检索号 | BAG70264.1 |
| 分子量 | 17.2 kDa |
| 表观分子量 | 16 kDa |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 0.1 EU/μg |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 5% sucrose, 0.02% Tween 80, pH 8.0. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 背景介绍 | Fibroblast Growth Factors (FGF) are a family of heparin-binding secreted Proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule. |
相关文献
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期刊：CURRENT MOLECULAR MEDICINE
DOI：10.2174/1566524023666230324135330
影响因子：2.500
相关产品： EGF/Urogastrone/URG, Human, Recombinant, FGF-2/bFGF/FGF-b (K128N), Human, Recombinant, BCA Protein Colorimetric Assay Kit
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期刊：APOPTOSIS
DOI：10.1007/s10495-022-01789-y
影响因子：5.600
相关产品： PC-9 Cells Line, A549 [A-549] Cells Line, NCI-H1299 Cells Line, NCI-H1975 Cells Line, BEAS-2B Cells Line, RPMI-1640, BEAS-2B Cells Complete Medium, EGF/Urogastrone/URG, Human, Recombinant, FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
