500μg / 50μg / 10μg / 1mg

Human

E.coli

MKNLNPKKFSIHDQDHKVLVLDSGNLIAVPDKNYIRPEIFFALASSLSSASAEKGSPILLGVSKGEFCLYCDKDKGQSHPSLQLKKEKLMKLAAQKESARRPFIFYRAQVGSWNMLESAAHPGWFICTSCNCNEPVGVTDKFENRKHIEFSFQPVCKAEMSPSEVSD with polyhistidine tag at the C-terminus.

MKNLNPKKFSIHDQDHKVLVLDSGNLIAVPDKNYIRPEIFFALASSLSSASAEKGSPILLGVSKGEFCLYCDKDKGQSHPSLQLKKEKLMKLAAQKESARRPFIFYRAQVGSWNMLESAAHPGWFICTSCNCNEPVGVTDKFENRKHIEFSFQPVCKAEMSPSEVSD with polyhistidine tag at the C-terminus.

Q9NZH6.1

19.49 kDa

22 kDa

His-tag at the C-terminus

Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 0.9 μg/mL.

> 95% as determined by SDS-PAGE. Ni-NTA chromatography.

< 0.1 EU/μg

The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0.

It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.

Ambient temperature or ice pack.

12个月

Decreasing synthesis of IL-37 in PBMCs will cause increasing production of pro-inflammatory cytokines such as IL-1 alpha, IL-1 beta, IL-6 and TNF-alpha. The IL-37 work as an innate inflammatory inhibitor is also proved by its high expression in synovial tissue from rheumatoid arthritis patients. Full length IL-37 locates mainly in the cytoplasm, and it can translocate to the nucleus after activation through cleavage by CASP1, then exerts its activity by directly interacting with SMAD3.