英文名称：FGF-2/bFGF/FGF-b, Human, Recombinant
货号：GPCK009
价格： ￥3480.00 ￥1080.00 ￥420.00 ￥4280.00
产品详情
产品信息
| 产品规格 | 500μg / 50μg / 10μg / 1mg |
| 别名 | HBGF-2;Prostatropin;Fibroblast Growth Factor 2;FGF-2;Basic Fibroblast Growth Factor;bFGF;Heparin-Binding Growth Factor 2;Fgf2;Fgf-2 |
| 表达宿主 | E.coli |
| 序列信息 | AAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS with polyhistidine tag at the N-terminus. |
| 检索号 | P09038.3 |
| 分子量 | 18.1 kDa |
| 表观分子量 | 17 kDa |
| 标签 | His-tag at the N-terminus |
| 生物活性 | Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant human FGF-2 is approximately > 5 × 10^5 IU/mg. |
| 纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | < 0.1 EU/μg |
| 制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 0.01% sarkosyl in 1 × PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 背景介绍 | FGF2, also known as a basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene. FGF2 has been shown in preliminary animal studies to protect the heart from injury associated with a heart attack, reducing tissue death and promoting improved function after reperfusion. FGF-2 (bFGF) are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth, and invasion. Additionally, FGF-2 (bFGF) is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems. |
相关文献
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期刊：CURRENT MOLECULAR MEDICINE
DOI：10.2174/1566524023666230324135330
影响因子：2.500
相关产品： EGF/Urogastrone/URG, Human, Recombinant, FGF-2/bFGF/FGF-b (K128N), Human, Recombinant, BCA Protein Colorimetric Assay Kit
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期刊：APOPTOSIS
DOI：10.1007/s10495-022-01789-y
影响因子：5.600
相关产品： PC-9 Cells Line, A549 [A-549] Cells Line, NCI-H1299 Cells Line, NCI-H1975 Cells Line, BEAS-2B Cells Line, RPMI-1640, BEAS-2B Cells Complete Medium, EGF/Urogastrone/URG, Human, Recombinant, FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
