英文名称：BMP-4/BMP-2B/DVR4, Mouse, Recombinant
货号：GPCK308
价格： ￥1680.00 ￥680.00
产品详情
50μg / 10μg
物种Mouse
表达宿主E.coli
PHMKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCRwith polyhistidine tag at the C-terminus.
序列信息MKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCRwith polyhistidine tag at the C-terminus.
检索号P21275
分子量12.88 kDa
表观分子量11-17 kDa
标签His-tag at the C-terminus
生物活性Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 10 ng/mL. The specific activity of recombinant mouse BMP-4 is > 1 × 10^5 IU/mg.
纯度> 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素< 0.1 EU/μg
制剂The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium carbonate, pH 4.5.
复融It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件Ambient temperature or ice pack.
有效期12个月
背景介绍Bone morphogenetic protein 4 is a protein that in humans is encoded by BMP4 gene. BMP4 is found on chromosome 14q22-q23. BMP4 is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. BMP4 is highly conserved evolutionarily. BMP4 is found in early embryonic development in the ventral marginal zone and in the eye, heart blood and otic vesicle.
