产品详情
产品信息
| 产品形态 | 粉末 |
| 产品规格 | 10μg |
| 表达宿主 | E.coli |
| 序列信息 | MEIPMSTVVKETLTQLSAHRALLTSNETMRLPVPTHKNHQLCIGEIFQGLDILKNQTVRGGTVEMLFQNLSLIKKYIDRQKEKCGEERRRTRQFLDYLQEFLGVMSTEWAMEG with polyhistidine tag at the C-terminus. |
| 检索号 | P04401.1 |
| 分子量 | 13.93 kDa |
| 表观分子量 | 11-17 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant mouse IL-5 is > 5 × 10^6 IU/mg. |
| 纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | < 0.1 EU/μg |
| 制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 背景介绍 | Interleukin 5 (IL-5) is an interleukin produced by type-2 T helper cells and mast cells. Through binding to the interleukin-5 receptor, interleukin 5 stimulates B cell growth and increases immunoglobulin secretion-primarily IgA. It is also a key mediator in eosinophil activation. |
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