英文名称：TNF-α/ TNFA/ TNFSF2, Human, Recombinant
货号：GPCK050
价格： ￥4880.00 ￥1780.00 ￥980.00 ￥480.00 ￥7080.00
产品详情
产品信息
| 产品规格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 表达宿主 | E.coli |
| 序列信息 | Val77-Leu233 |
| 检索号 | P01375 |
| 分子量 | 17.5 kDa |
| 表观分子量 | 16 kDa |
| 生物活性 | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/mL. |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 0.1 EU/μg |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 6.0. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 背景介绍 | TNFα is a homotrimer with a subunit molecular mass of 17 kD Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease. |
相关文献
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期刊：Journal of Inflammation Research
影响因子：4.1
相关产品： CCK8, TUNEL In Situ (Elab Fluor® 488), Human BNP ELISA Kit, TNF-α/ TNFA/ TNFSF2, Human, Recombinant, AC16 Cells Line
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期刊：LAB ON A CHIP
影响因子：6.1
相关产品： Human Umbilical Vein Endothelial cells, TNF-α/ TNFA/ TNFSF2, Human, Recombinant, Calcein-AM/PI Double Staining Kit
