英文名称：IL-1β/IL1F2, Human, Recombinant
货号：GPCK047
价格： ￥11880.00 ￥2280.00 ￥780.00 ￥17280.00
产品详情
产品信息
| 产品规格 | 500μg / 50μg / 10μg / 1mg |
| 表达宿主 | E.coli |
| 序列信息 | MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS with polyhistidine tag at the C-terminus. |
| 检索号 | P01584.2 |
| 分子量 | 18.48 kDa |
| 表观分子量 | 17 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is < 10 pg/mL. The specific activity of recombinant human IL-1 beta is approximately > 1.5 × 10^8 IU/mg.Measure by its ability to induce IL-8 secretion in HT29 cells. The ED50 for this effect is 1.8-5.1 ng/mL. |
| 纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | < 0.1 EU/μg |
| 制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 背景介绍 | Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. |
相关文献
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期刊：IMMUNOLOGICAL INVESTIGATIONS
DOI：10.1080/08820139.2023.2183135
影响因子：2.800
相关产品： IL-1β/IL1F2, Human, Recombinant, IL-1β/IL1F2, Mouse, Recombinant
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期刊：Molecular And Cellular Probes
影响因子：3.300
相关产品： DMEM/F12, Penicillin-Streptomycin Solution, 100×, Fetal Bovine Serum, IL-1β/IL1F2, Human, Recombinant
