产品详情
产品信息
产品规格 | 50μg / 10μg |
表达宿主 | E.coli |
序列信息 | NWIDVRYDLEKIESLIQSIHIDTTLYTDSDFHPSCKVTAMNCFLLELQVILHEYSNMTLNETVRNVLYLANSTLSSNKNVAESGCKECEELEEKTFTEFLQSFIRIVQMFINTS with polyhistidine tag at the N-terminus. |
检索号 | P48346.1 |
分子量 | 14.2 kDa |
表观分子量 | 11-14 kDa |
标签 | His-tag at the N-terminus |
生物活性 | Measure by its ability to induce CTLL-2 cells proliferation.The ED50 for this effect is < 10 ng/mL. The specific activity of recombinant mouse IL-15 is approximately > 1× 10^5 IU/mg. |
纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
内毒素 | < 0.1 EU/μg |
制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
运输条件 | Ambient temperature or ice pack. |
有效期 | 12个月 |
背景介绍 | Interleukin-15 (IL-15) is a cytokine with structural similarity to Interleukin-2 (IL-2). Like IL-2, IL-15 binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. |
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