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TGFβ 1/TGFB1, Human, Recombinant-1

英文名称：TGFβ 1/TGFB1, Human, Recombinant

货号：GPCK354

价格： ￥1680.00 ￥680.00

规格：
50μg 10μg
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产品信息

产品规格 50μg / 10μg
表达宿主 E.coli
序列信息 MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS with polyhistidine tag at the C-terminus.
检索号 P01137.2
分子量 13.7 kDa
表观分子量 13 kDa
标签 His-tag at the C-terminus
生物活性 Measure by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells.The ED50 for this effect is < 0.1 ng/mL. The specific activity of recombinant human TGF beta 1 is approximately > 5 × 10^7 IU/mg. Measure by its ability to induce proliferation in MCF-7 cells. The ED50 for this effect is < 3.2 ng/mL.
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene.
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