产品详情
产品信息
| 产品规格 | 10μg |
| 表达宿主 | E.coli |
| 序列信息 | MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA with polyhistidine tag at the C-terminus. |
| 检索号 | P14174.4 |
| 分子量 | 13.28 kDa |
| 表观分子量 | 10 kDa |
| 标签 | His-tag at the C-terminus |
| 纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | < 0.1 EU/μg |
| 制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 背景介绍 | Macrophage migration inhibitory factor (MIF or MMIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein that in humans is encoded by the MIF gene. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread and disease-free survival. |
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