产品详情
产品信息
产品规格 | 10μg |
表达宿主 | E.coli |
序列信息 | MECHIKDKEGKAYESVLMISIDELDKMTGTDSNCPNNEPNFFRKHVCDDTKEAAFLNRAARKLKQFLKMNISEEFNVHLLTVSQGTQTLVNCTSKEEKNVKEQKKNDACFLKRLLREIKTCWNKILKGSI with polyhistidine tag at the C-terminus. |
检索号 | P10168.1 |
分子量 | 15.84 kDa |
表观分子量 | 16 kDa |
标签 | His-tag at the C-terminus |
生物活性 | Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC). The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant mouse IL-7 is > 5 × 10^6 IU/mg. |
纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
内毒素 | < 0.1 EU/μg |
制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
运输条件 | Ambient temperature or ice pack. |
有效期 | 12个月 |
背景介绍 | Interleukin 7 (IL-7) is a protein that in humans is encoded by the IL7 gene. IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells. It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. |
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