请输入关键词！

说明书
一键复制产品信息
TNF-α/TNFA/TNFSF2, Mouse, Recombinant-1

英文名称：TNF-α/TNFA/TNFSF2, Mouse, Recombinant

货号：GPCK055

价格： ￥12880.00 ￥2280.00 ￥780.00 ￥18880.00

规格：
500μg 50μg 10μg 1mg
产品详情

产品信息

产品形态 粉末
产品规格 500μg / 50μg / 10μg / 1mg
表达宿主 E.coli
序列信息 MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYL DFAESGQVYFGVIAL with polyhistidine tag at the C-terminus.
检索号 P06804.29 (Mouse), P16599.1 (Rat)
分子量 18.20 kDa
表观分子量 16 kDa
标签 His-tag at the C-terminus
生物活性 Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is < 40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately > 2.5× 10^7 IU/mg.
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 TNF-α is a kind of pleiotropic pro-inflammatory cytokine. It is secreted by various cells, such as adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. Proteolysis of the integral membrane precursor form of TNF-α from cells soluble can release homotrimeric TNF-α. TNF-α can bind with some TNF-α receptors induces apoptosis, besides, also trigger other responses depending on cell type, receptor expression, and signal transduction status. TNF-α participate in the inflammatory response.
产品推荐
-产品推荐-3
-产品推荐-3
-产品推荐-3
-产品推荐-3
-产品推荐-3
-产品推荐-3
联系我们
sales@procell.com.cn
400-999-2100
相关推荐
×
确定