产品详情
10μg
物种Swine
表达宿主E.coli
PHMNSYSECPPSHDGYCLHGGVCMYIEAVDSYACNCVFGYVGERCQHRDLKWWELR with polyhistidine tag at the C-terminus.
序列信息MNSYSECPPSHDGYCLHGGVCMYIEAVDSYACNCVFGYVGERCQHRDLKWWELR with polyhistidine tag at the C-terminus.
检索号Q00968.2
分子量7.1 kDa
表观分子量below 11 kDa
标签His-tag at the C-terminus
纯度> 95% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素< 0.1 EU/μg
制剂The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0.
复融It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件Ambient temperature or ice pack.
有效期12个月
背景介绍Epidermal growth factor (EGF) stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is a 6-kDa protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
