英文名称：TNF-α/TNFA/TNFSF2, Rat, Recombinant
货号：GPCK103
价格： ￥11880.00 ￥2280.00 ￥780.00 ￥17280.00
产品详情
500μg / 50μg / 10μg / 1mg
物种Rat
表达宿主E.coli
PHLeu80-Leu235
序列信息Leu80-Leu235
检索号P16599
分子量17.4 kDa
表观分子量14 kDa
纯度> 95% as determined by reducing SDS-PAGE.
内毒素< 0.1 EU/μg
制剂Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复融Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存条件Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months.
运输条件Ambient temperature or ice pack.
有效期12个月
背景介绍Tumor necrosis factor alpha (TNF-alpha, TNFSF2) is the prototypic Ligand of the TNF superfamily. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key Cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.
