英文名称:SW620
货号:GCL-0225
价格: ¥1600.00 ¥1600.00
产品详情
产品信息
物种 | Human |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:Leibovitz's L-15[GPM151010]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
培养方案B(可选) |
生长培养基:DMEM[GPM150210]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
注意事项 |
|
参考资料(来源文献)
背景描述 | SW620细胞是从一个51岁男性白人组织中分离得到的,由A·Leibovitz等从一个淋巴结建株。SW620细胞主要由无绒毛的小园球细胞和双极细胞组成;SW620细胞仅合成少量癌胚抗原(CEA),在裸鼠中有高度的致瘤性。 |
年龄(性别) | 51Y |
组织来源 | 结直肠腺癌,来自转移淋巴结 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
抗原表达情况 | Blood Type A; Rh+ |
基因表达情况 | Carcinoembryonic antigen (CEA) 0.15 ng/10⁶ cells/10 days; transforming growth factor alpha; matrilysin. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells). |
保藏机构 | ATCC; CCL-227 ECACC; 87051203 |
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