文献(1)
说明书

英文名称:SCF/c-Kit/KIT ligand, Human, Recombinant
货号:GPCK019
价格: ¥8880.00 ¥1480.00 ¥680.00 ¥13880.00
规格:
500μg
50μg
10μg
1mg
产品详情
产品信息
产品规格 | 500μg / 50μg / 10μg / 1mg |
表达宿主 | E.coli |
序列信息 | MEGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA with polyhistidine tag at the C-terminus |
检索号 | P21583.1 |
分子量 | 19.4 kDa |
表观分子量 | 21 kDa |
标签 | His-tag at the C-terminus |
生物活性 | Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 5 ng/mL. The specific activity of recombinant human SCF is > 5 × 10^5 IU/mg. |
纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
内毒素 | < 0.1 EU/μg |
制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
运输条件 | Ambient temperature or ice pack. |
有效期 | 12个月 |
背景介绍 | Stem Cell Factor (SCF) is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. SCF is a growth factor important for proliferation, and differentiation of hematopoietic stem cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid). |
相关文献
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期刊:JOURNAL OF CLINICAL LABORATORY ANALYSIS
影响因子:3.124
相关产品: SCF/c-Kit/KIT ligand, Human, Recombinant, IL-6/IFN-β2/BSF-2, Human, Recombinant, 293T [HEK-293T] Cells Line, Human Skin Mast Cell Complete Medium
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