
英文名称:GM-CSF/CSF2/CSF, Human, Recombinant
货号:GPCK035
价格: ¥9680.00 ¥2780.00 ¥880.00 ¥13880.00
规格:
500μg
50μg
10μg
1mg
产品详情
产品信息
产品形态 | 粉末 |
产品规格 | 500μg / 50μg / 10μg / 1mg |
表达宿主 | E.coli |
序列信息 | APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE with polyhistidine tag at the N-terminus. |
检索号 | P04141.1 |
分子量 | 15.4 kDa |
表观分子量 | 15 kDa |
标签 | His-tag at the N-terminus |
生物活性 | Measure by its ability to induce TF-1 cells proliferation.The ED50 for this effect is < 80 pg/mL. The specific activity of recombinant human GM-CSF is approximately > 1 × 10^7 IU/mg. |
纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
内毒素 | < 0.1 EU/μg |
制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
运输条件 | Ambient temperature or ice pack. |
有效期 | 12个月 |
背景介绍 | Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF also plays a role in embryonic development by functioning as an embryokine produced by reproductive tract. |
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