英文名称：NCI-H1299
货号：GCL-0165
价格： ￥1600.00 ￥1600.00
产品详情
产品信息
| 物种 | Human |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：无血清非程序冻存液（GPB180438）/通用血清型程序冻存液（GPB180436） 温度：液氮 |
| 培养方案A（默认） |
生长培养基：RPMI-1640[GPM150110]+10% FBS[163210]+1% P/S[GPB180120] 培养条件： 温度：37℃ |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料（来源文献）
| 背景描述 | NCI-H1299细胞来源于一个淋巴结转移，患者接受了初期放疗。NCI-H1299细胞均一性的部分缺失p53蛋白，并缺少p53蛋白表达。NCI-H1299细胞可以合成0.1 pmol/毫克蛋白的NMB蛋白，而不合成促胃液释放肽（GRP）。 |
| 年龄（性别） | 43Y |
| 组织来源 | 肺癌：非小细胞肺癌；转移灶：淋巴结 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
| 基因表达情况 | These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. neuromedin B, The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B. |
| 保藏机构 | ATCC; CRL-5803 |
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