英文名称:NCI-H1299
货号:GCL-0165
价格: ¥1600.00 ¥1600.00
产品详情
产品信息
物种 | Human |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:无血清非程序冻存液(GPB180438)/通用血清型程序冻存液(GPB180436) 温度:液氮 |
培养方案A(默认) |
生长培养基:RPMI-1640[GPM150110]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | NCI-H1299细胞来源于一个淋巴结转移,患者接受了初期放疗。NCI-H1299细胞均一性的部分缺失p53蛋白,并缺少p53蛋白表达。NCI-H1299细胞可以合成0.1 pmol/毫克蛋白的NMB蛋白,而不合成促胃液释放肽(GRP)。 |
年龄(性别) | 43Y |
组织来源 | 肺癌:非小细胞肺癌;转移灶:淋巴结 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
基因表达情况 | These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. neuromedin B, The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B. |
保藏机构 | ATCC; CRL-5803 |
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