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VEGF165/VPF, Mouse, Recombinant-1

英文名称：VEGF165/VPF, Mouse, Recombinant

货号：GPCK306

价格： ￥1680.00 ￥680.00

规格：
50μg 10μg
产品详情

产品信息

产品规格 50μg / 10μg
表达宿主 E.coli
序列信息 MAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPENHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR with polyhistidine tag at the C-terminus.
检索号 Q00731.2 (Mouse), P16612.2 (Rat)
分子量 20.22 kDa
表观分子量 18 kDa
标签 His-tag at the C-terminus
生物活性 Measure by its ability to induce proliferation in HUVEC cells. The ED50 for this effect is < 3 ng/mL.
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha.
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