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FGF-2/bFGF/FGF-b, Mouse, Recombinant-1

英文名称：FGF-2/bFGF/FGF-b, Mouse, Recombinant

货号：GPCK011

价格： ￥4880.00 ￥1080.00 ￥420.00 ￥6680.00

规格：
500μg 50μg 10μg 1mg
产品详情

产品信息

产品规格 500μg / 50μg / 10μg / 1mg
别名 HBGF-2;Prostatropin;Fibroblast Growth Factor 2;FGF-2;Basic Fibroblast Growth Factor;bFGF;Heparin-Binding Growth Factor 2;Fgf2;Fgf-2
表达宿主 E.coli
序列信息 ALPEDGGAAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS with polyhistidine tag at the N-terminus.
检索号 P15655.1 (Mouse), P13109.1 (Rat)
分子量 17.2 kDa
表观分子量 19 kDa
标签 His-tag at the N-terminus
生物活性 Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 1.5 ng/mL. The specific activity of recombinant mouse FGF-2 is approximately > 1 × 10^6 IU/mg.
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 0.01% sarkosyl in 1 × PBS, pH 8.0.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 FGF2, also known as a basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene. FGF2 has been shown in preliminary animal studies to protect the heart from injury associated with a heart attack, reducing tissue death and promoting improved function after reperfusion. FGF-2 (bFGF) are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth, and invasion. Additionally, FGF-2 (bFGF) is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.
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