产品详情
10μg
物种Human
表达宿主E.coli
PHTPVVRKGRCSCISTNQGTIHLQSLKDLKQFAPSPSCEKIEIIATLKNGVQTCLNPDSADVKELIKKWEKQVSQKKKQKNGKKHQKKKVLKVRKSQRSRQKKTT with polyhistidine tag at the N-terminus.
序列信息TPVVRKGRCSCISTNQGTIHLQSLKDLKQFAPSPSCEKIEIIATLKNGVQTCLNPDSADVKELIKKWEKQVSQKKKQKNGKKHQKKKVLKVRKSQRSRQKKTT with polyhistidine tag at the N-terminus.
检索号Q07325.1
分子量12.53 kDa
表观分子量18 kDa
标签His-tag at the N-terminus
生物活性Measure by its ability to chemoattract BaF3 cells transfected with mouse CXCR3. The ED50 for this effect is < 0.5 μg/mL.
纯度> 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素< 0.1 EU/μg
制剂The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4.
复融It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件Ambient temperature or ice pack.
有效期12个月
背景介绍CXCL9, also named Monokine, is a member of the CXC chemokine family and is induced by gamma interferon (MIG). Following induced by IFN-gamma, this chemokine can attract T-cells. CXCL9 has close relationship with two other CXC chemokines named CXCL10 and CXCL11, additionally they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. CXCL9 is also a cytokine that affects the growth, movement, or activation state of cells participating in immune and inflammatory response and work as a chemoattractant of activated T-cells.
