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FasL/TNFSF6/CD95L/CD178, Human, Recombinant-1

英文名称：FasL/TNFSF6/CD95L/CD178, Human, Recombinant

货号：GPCK291

价格： ￥680.00

规格：
10μg
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产品信息

产品规格 10μg
表达宿主 E.coli
序列信息 QIGHPSPPPEKKELRKVAHLTGKSNSRSMPLEWEDTYGIVLLSGVKYKKGGLVINETGLYFVYSKVYFRGQSCNNLPLSHKVYMRNSKYPQDLVMMEGKMMSYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESQTFFGLYKL with polyhistidine tag and sumo tag at the N-terminus
检索号 P48023.1
分子量 17.31 kDa
表观分子量 30-35 kDa
标签 His-SUMO tag at the N-terminus
生物活性 Measure by its ability to induce apoptosis in Jurkat cells. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant human FasL is > 1 × 10^6 IU/mg.
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 FasL is a member of the TNF superfamily, and is mainly expressed on the cell surface of activated T cells. FasL induces apoptosis in Fas-bearing cells by binding to Fas Receptor. FasL has the ability to leads to down-regulation of the immune response through killing T cells and activated B cells. The mechanism of Fas-induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death.
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