英文名称:RAW 264.7
货号:GCL-0190
价格: ¥1600.00 ¥1600.00
产品详情
产品信息
物种 | Mouse |
生长特性 | 贴壁细胞,不用胰酶消化 |
细胞形态 | 不规则形 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:DMEM[GPM150210]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 1:2-1:8 |
推荐换液频率 | 2-3次/周 |
注意事项 |
|
参考资料(来源文献)
背景描述 | RAW 264.7细胞源自Abelson鼠科白血病病毒诱导的肿瘤;sIg-、Ia-抗原、Thy-1.2表面抗原阴性。RAW 264.7细胞不分泌可检测到的病毒颗粒,XC斑点形成试验阴性。RAW 264.7细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖,并且可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞。LPS或PPD处理2天可诱导RAW 264.7细胞分解红血球,但对肿瘤靶细胞无作用。 |
年龄(性别) | Adult |
组织来源 | Abelson鼠科白血病病毒诱导的肿瘤;单核细胞;巨噬细胞 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 白血病细胞 |
生物安全等级 | BSL-2 |
抗原表达情况 | H-2d |
基因表达情况 | lysozyme, H-2d; Tested and found negative for ectromelia virus (mousepox). |
受体表达情况 | complement (C3) |
保藏机构 | ATCC; TIB-71 ECACC; 91062702 |
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