英文名称:DMS 114
货号:GCL-0752
价格: ¥1500.00 ¥1500.00
产品详情
产品信息
物种 | Human |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:无血清非程序冻存液(GPB180438)/通用血清型程序冻存液(GPB180436) 温度:液氮 |
培养方案A(默认) |
生长培养基:Waymouth's MB 752/1[GPM151215]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. |
年龄(性别) | 68Y |
组织来源 | 肺 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
抗原表达情况 | Leu 7 +; My23 +; CD11b + |
基因表达情况 | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) |
致瘤性 | Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
保藏机构 | ATCC; CRL-2066 |
相关文献
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期刊:Advanced Science
影响因子:14.3
相关产品: Cell Ferrous Iron Colorimetric Assay Kit, Fetal Bovine Serum, RAW 264.7 Cells Line, DMS 114 Cells Line, NCI-H446 [H446] Cells Line
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期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI:10.1016/j.taap.2024.116915
影响因子:3.8
相关产品: NCI-H446 [H446] Cells Line, NCI-H146 [H146] Cells Line, NCI-H209 [H209] Cells Line, DMS 114 Cells Line, H1688 Cells Line, Annexin V-APC/7-AAD, Cell Cycle (Red Fluorescence)
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期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI:10.1016/j.taap.2024.117005
影响因子:3.3
相关产品: Annexin V-APC/7-AAD , Cell Cycle (Red Fluorescence), NCI-H146 [H146] Cells Line, NCI-H446 [H446] Cells Line, NCI-H209 [H209] Cells Line, NCI-H1688 Cells Line, DMS 114 Cells Line