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  • DMS 114-1
DMS 114-1

英文名称:DMS 114

货号:GCL-0752

价格: ¥1500.00 ¥1500.00

规格:
1×10^6Cells/T25 1×10^6Cells/Vial×2Vials
产品详情

产品信息

物种 Human
生长特性 贴壁细胞
细胞形态 上皮细胞样
冻存条件 冻存液:无血清非程序冻存液(GPB180438)/通用血清型程序冻存液(GPB180436)
温度:液氮
培养方案A(默认) 生长培养基:Waymouth's MB 752/1[GPM151215]+10% FBS[163210]+1% P/S[GPB180120]
培养条件: 温度:37℃
推荐传代比例 1:2-1:4
推荐换液频率 2-3次/周
参考资料(来源文献)
背景描述 The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin.
年龄(性别) 68Y
组织来源
细胞类型 肿瘤细胞
肿瘤类型 肺癌细胞
生物安全等级 BSL-1
抗原表达情况 Leu 7 +; My23 +; CD11b +
基因表达情况 adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP)
致瘤性 Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
保藏机构 ATCC; CRL-2066
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