PC-12 (Undifferentiated) -

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PC-12 (Undifferentiated)-1

英文名称：PC-12 (Undifferentiated)

货号：GCL-0412

规格：

1×10^6Cells/T25 1×10^6Cells/Vial×2Vials

产品详情

产品信息

物种	Rat
生长特性	聚团悬浮，疏松贴壁
细胞形态	圆形
冻存条件	冻存液：无血清非程序冻存液（GPB180438）/通用血清型程序冻存液（GPB180436）温度：液氮
培养方案A（默认）	生长培养基：RPMI-1640[GPM150110]+15% HS[163215]+5% FBS[163210]+1% P/S[GPB180120] 培养条件：温度：37℃
推荐传代比例	1:2-1:4
推荐换液频率	2-3次/周
注意事项	1.PC-12(Undifferentiated)细胞正常生长状态为悬浮聚团，存在聚团细胞贴壁情况。若需要用NGF诱导时，需要使用多聚-L-赖氨酸溶液（货号PB180523）包被培养瓶后促使细胞贴壁。 2.Undifferentiated、Low differentiation和High differentiation的区别：Undifferentiated的PC-12从形态上看是圆形的，聚团生长的漂浮细胞。 Low differentiation的成多角形，有较短的突起。 High differentiation的细胞有多个突起，突起数目不等，突触较长，类似神经元轴突。3.Low differentiation和High differentiation是在Undifferentiated的PC-12基础上诱导产生的，Low differentiation和High differentiation指的与神经细胞表型的相似程度，High differentiation更接近神经细胞表型。

参考资料（来源文献）

背景描述	PC-12(Undifferentiated)细胞是来自能移植的雄性大鼠肾上腺嗜铬细胞瘤。PC-12(Undifferentiated)细胞表达神经生长因子（NGF）受体，NGF可诱导产生神经表型。PC-12(Undifferentiated)细胞不合成肾上腺素。
组织来源	肾上腺嗜铬细胞瘤
细胞类型	肿瘤细胞
肿瘤类型	嗜铬细胞瘤细胞

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