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文献(3) 说明书
  • OE19-1
OE19-1

英文名称:OE19

货号:GCL-0754

价格: ¥1800.00 ¥1800.00

规格:
1×10^6Cells/T25 1×10^6Cells/Vial×2Vials
产品详情

产品信息

物种 Human
生长特性 贴壁细胞
细胞形态 上皮细胞样
冻存条件 冻存液:55% 基础培养基+40% FBS+5% DMSO
温度:液氮
培养方案A(默认) 生长培养基:RPMI-1640[GPM150110]+10% FBS[163210]+1% P/S[GPB180120]
培养条件: 温度:37℃
推荐传代比例 1:2-1:4
推荐换液频率 2-3天/次
参考资料(来源文献)
背景描述 OE19, also known as JROECL19, was originally one of five permanent human oesophageal adenocarcinoma (EAC) cell lines established in 1993. It is from a stage III tumour of a 72 year old male patient1 and deposited in ECACC by Drs J C Rockett/A Morris, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital. Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines but large cell lung cancer cell line H460, colorectal adenocarcinoma cell line SW620, and gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs2. All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC.
年龄(性别) 72Y
组织来源 食管癌
细胞类型 肿瘤细胞
肿瘤类型 食管癌细胞
生物安全等级 BSL-1
致瘤性 裸小鼠致癌
保藏机构 ECACC; 96071721
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