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β-NGF/NGF/NGFB/NGF-β, Human, Recombinant-1

英文名称：β-NGF/NGF/NGFB/NGF-β, Human, Recombinant

货号：GPCK070

价格： ￥8880.00 ￥1180.00 ￥420.00 ￥11880.00

规格：
500μg 50μg 10μg 1mg
产品详情

产品信息

产品规格 500μg / 50μg / 10μg / 1mg
表达宿主 E.coli
序列信息 MSSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVRRA with polyhistidine tag at the C-terminus.
检索号 CAA36832
分子量 14.43 kDa
表观分子量 11 kDa
标签 His-tag at the C-terminus
生物活性 Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.7 ng/mL. The specific activity of recombinant human beta-NGF is > 1 × 10^6 IU/mg.
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 < 0.1 EU/μg
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution,store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
背景介绍 Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. NGF-β acts through its receptor β-NGFR and is involved in the development and maintenance of the sensory and sympathetic nervous systems. NGF-β also is also involved in the growth, differentiation, and survival of B lymphocytes. Human, mouse and rat proteins show cross-reactivity.
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