英文名称：NCI-H146 [H146]
货号：GCL-0693
价格： ￥1800.00 ￥1800.00
产品详情
产品信息
| 物种 | Human |
| 生长特性 | 悬浮细胞，聚团生长 |
| 细胞形态 | 淋巴母细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基：RPMI-1640[GPM150110]+10% FBS[163210]+1% P/S[GPB180120] 培养条件： 温度：37℃ |
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 |
|
参考资料（来源文献）
| 背景描述 | Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected. |
| 年龄（性别） | 59Y |
| 组织来源 | 肺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 基因表达情况 | vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes - |
| 保藏机构 | ATCC; HTB-173 |
相关文献
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期刊：TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI：10.1016/j.taap.2024.116915
影响因子：3.8
相关产品： NCI-H446 [H446] Cells Line, NCI-H146 [H146] Cells Line, NCI-H209 [H209] Cells Line, DMS 114 Cells Line, H1688 Cells Line, Annexin V-APC/7-AAD, Cell Cycle (Red Fluorescence)
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期刊：TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI：10.1016/j.taap.2024.117005
影响因子：3.3
相关产品： Annexin V-APC/7-AAD , Cell Cycle (Red Fluorescence), NCI-H146 [H146] Cells Line, NCI-H446 [H446] Cells Line, NCI-H209 [H209] Cells Line, NCI-H1688 Cells Line, DMS 114 Cells Line
