NCI-H1299 -

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NCI-H1299-1

英文名称：NCI-H1299

货号：GCL-0165

规格：

1×10^6Cells/T25 1×10^6Cells/Vial×2Vials

产品详情

产品信息

物种	Human
生长特性	贴壁细胞
细胞形态	上皮细胞样
冻存条件	冻存液：无血清非程序冻存液（GPB180438）/通用血清型程序冻存液（GPB180436）温度：液氮
培养方案A（默认）	生长培养基：RPMI-1640[GPM150110]+10% FBS[163210]+1% P/S[GPB180120] 培养条件：温度：37℃
推荐传代比例	1:3-1:6
推荐换液频率	2-3次/周

参考资料（来源文献）

背景描述	NCI-H1299细胞来源于一个淋巴结转移，患者接受了初期放疗。NCI-H1299细胞均一性的部分缺失p53蛋白，并缺少p53蛋白表达。NCI-H1299细胞可以合成0.1 pmol/毫克蛋白的NMB蛋白，而不合成促胃液释放肽（GRP）。
年龄（性别）	43Y
组织来源	肺癌：非小细胞肺癌；转移灶：淋巴结
细胞类型	肿瘤细胞
肿瘤类型	肺癌细胞
生物安全等级	BSL-1
基因表达情况	These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. neuromedin B, The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B.
保藏机构	ATCC; CRL-5803

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