英文名称:LoVo
货号:GCL-0144
价格: ¥1400.00 ¥1400.00
产品详情
产品信息
物种 | Human |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:Ham's F-12K[GPM150910]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 1:3-1:8 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | LoVo细胞是于1971年从诊断为结肠腺癌的56岁男性白人的一个转移到左锁骨上区的肿瘤结节建系而来。癌基因检测表明,LoVo细胞C-myc、K-ras、H-ras、N-ras、Myb、sis和fos的表达呈阳性;癌基因N-myc的表达未做检测。LoVo细胞在裸鼠中能成瘤,与107细胞联合皮下接种5只裸鼠21天后全部成瘤。LoVo表达肿瘤特异的核基质蛋白蛋白CC-3和CC-4。 |
年龄(性别) | 56Y |
组织来源 | 直结肠腺癌,转移位置:左锁骨上区 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
抗原表达情况 | HLA A11, B15, B17, Cw1, Cw3; Blood Type B |
基因表达情况 | carcinoembryonic antigen (CEA) 908 ng/10⁶ cells/10 days. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3. |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells). |
保藏机构 | ATCC; CCL-229 BCRC; 60148 BCRJ; 0332 CCLV; CCLV-RIE 1151 DSMZ; ACC-350 ECACC; 87060101 |
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