High Sensitivity Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称:高敏型小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
货号:GEHSM0001
价格: ¥3200.00 ¥2200.00 ¥14400.00
产品详情
试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-1β浓度
产品应用ELISA
检测原理本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-1β浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-1β的浓度。
反应类型Sandwich-ELISA
反应时间3 h 30 min
反应性Mouse
检测方法比色法、ELISA、夹心法
检测范围3.13-200 pg/mL
灵敏度1.41 pg/mL
样本体积100 μL
样本类型血清、血浆或其他生物体液
特异性可检测样本中的小鼠IL-1β,且与其它类似物无明显交叉反应
精密度板内,板间变异系数均<10%
回收率80%-120%
储存条件2-8℃
有效期12个月
产品结果图
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