High Sensitivity Rat IL-10(Interleukin 10) ELISA Kit

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High Sensitivity Rat IL-10(Interleukin 10) ELISA Kit-1

中文名称：高敏型大鼠白介素10(IL-10)酶联免疫吸附测定试剂盒

货号：GEHSR0005

规格：

96 T 48 T 96 T × 5

产品详情

用途	试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中IL-10浓度
产品应用	ELISA
别称	Il10;Interleukin-10;CSIF;Cytokine synthesis inhibitory factor;Il-10
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-10会与包被抗体结合。后依次加入生物素化的抗大鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-10抗体与结合在包被抗体上的大鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。
反应类型	Sandwich-ELISA
反应时间	3 h 30 min
反应性	Rat
检测方法	比色法、ELISA、夹心法
检测范围	3.13-200 pg/mL
灵敏度	1.22 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的大鼠IL-10，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月

产品结果图

High Sensitivity Rat IL-10(Interleukin 10) ELISA Kit-产品结果图-1

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