Cell Mitochondrial Complex II Activity Assay Kit
中文名称:细胞线粒体呼吸链复合物II比色法测试盒
货号:GBQ242
价格: ¥2200.00 ¥1800.00
产品详情
检测原理 |
线粒体呼吸链复合物Ⅱ又称琥珀酸-辅酶Q还原酶,广泛存在于动物、植物、微生物和细胞的线粒体中,它催化三羧酸循环中的琥珀酸氧化成延胡索酸(富马酸),通过复合物中的基本单元结构比如硫铁蛋白等,使泛醌转化为还原形态。复合体Ⅱ的催化产物还原型辅酶Q可进一步还原2,6-二氯吲哚酚,2,6-二氯吲哚酚在600 nm有特征吸收峰,通过检测2,6-二氯吲哚酚的减少速率来计算该酶活性。
|
灵敏度 | 0.54 U/L |
检测范围 | 0.54-17.66 U/L |
精密度 | 批间差:3.0-6.0% | 批内差:2.0-5.0% |
样本量 | 20 μL(cell homogenate) |
检测时间 | 30 min |
保存条件 | 该产品可在-20°C避光条件下保存6个月。 |
有效期 | 6个月 |
产品结果图
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