英文名称:BT-474 [BT474]
货号:GCL-0040
价格: ¥1600.00 ¥1600.00
产品详情
产品信息
物种 | Human |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:RPMI-1640[GPM150110]+10 μg/mL Insulin[GPB180432]+2 mM L-glutamine[GPB180420]+20% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 |
|
参考资料(来源文献)
背景描述 | BT-474 [BT474]细胞是由E·Lasfargues和W·G·Coutinho从一个实心的乳腺浸润性导管癌中分离到的细胞株。 |
年龄(性别) | 60Y |
组织来源 | 乳腺导管癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in Amsterdam/IMR rats with regression in 10 days.Yes, in nude mice. |
保藏机构 | ATCC; CRL-7913 ATCC; HTB-20 BCRC; 60359 BCRJ; 0353 DSMZ; ACC-64 |
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