0.25%胰蛋白酶溶液(含EDTA,溶于PBS)

英文名称:0.25% Trypsin Solution, with EDTA, dissolved in PBS
货号:GPB180225
价格: ¥228.00 ¥68.00
产品详情
胰蛋白酶(Trypsin)是一种丝氨酸水解酶,它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段,水解细胞间的蛋白质,破坏细胞间的连接,从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关,在pH8.0和37℃时,胰酶的作用能力最强,因此使用胰酶时,应把握好浓度、温度和时间,以免消化过度造成细胞损伤。
一般常用胰酶的工作浓度为0.25%,而半贴壁细胞或对胰酶敏感的细胞常采用低浓度(0.05%)的胰酶进行细胞消化。由于EDTA能够螯合Ca²⁺和Mg²⁺,从而破坏细胞连接促进细胞的解离,因此在胰酶溶液中常常会加入一定量的EDTA混合使用,以增强解离效果。
500mL / 125mL
内毒素< 3 EU/mL
储存条件-5~-20℃,避光储存
运输条件低温
有效期12个月
注意事项1. 由于不同的组织或者细胞对胰酶的作用反应不一样,因此操作人员应根据实际情况,确定最佳消化时间;消化细胞时间不宜过长,否则会影响细胞贴壁和生长状况;<br/>2. 使用本产品时应注意无菌操作,避免污染;<br/>3. 不宜长时间放置于室温环境或2-8℃长期保存;<br/>4. 2-8℃中解冻,摇匀后使用,切忌反复冻融,用量较少时建议分装冻存;<br/>5. 本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
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