英文名称:U-937
货号:GCL-0239
价格: ¥1600.00 ¥1600.00
产品详情
产品信息
物种 | Human |
生长特性 | 悬浮细胞 |
细胞形态 | 单核细胞 |
冻存条件 |
冻存液:90% FBS+10% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:RPMI-1640[GPM150110]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 5×10⁵-1×10⁶ cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 |
|
参考资料(来源文献)
背景描述 | U-937细胞是由Sundstrom和Nilsson于1974年建立,取材于患组织细胞淋巴瘤病人的胸水。研究表明:U-937细胞能被人混合淋巴细胞培养上清,佛波脂、维生素D3、γ干扰素、肿瘤坏死因子和维甲酸诱导终末单核细胞分化。U-937细胞免疫球蛋白产物和EB病毒表达为阴性;U-937细胞表达Fas抗原且对TNF和抗-Fas抗体敏感。 |
年龄(性别) | 37Y |
组织来源 | 组织细胞淋巴瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 淋巴瘤细胞 |
生物安全等级 | BSL-1 |
基因表达情况 | lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA) |
受体表达情况 | complement (C3) |
保藏机构 | ATCC; CRL-1593ATCC; CRL-1593.2 DSMZ; ACC-5 ECACC; 85011440 |
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