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文献(3) 说明书
  • U-2932-1
U-2932-1

英文名称:U-2932

货号:GCL-0838

价格: ¥1800.00 ¥1800.00

规格:
1×10^6Cells/T25 1×10^6Cells/Vial×2Vials
产品详情

产品信息

物种 Human
生长特性 悬浮细胞
细胞形态 淋巴母细胞样,圆形,单个细胞,少数聚团
冻存条件 冻存液:55% 基础培养基+40% FBS+5% DMSO
温度:液氮
培养方案A(默认) 生长培养基:RPMI-1640[GPM150110]+10% FBS[163210]+1% P/S[GPB180120]
培养条件: 温度:37℃
推荐传代比例 1:3-1:4
推荐换液频率 2-3次/周
参考资料(来源文献)
背景描述 established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq). Also, please note: the cell line U-2932 harbours two distinct subpopulations as visible by surface staining of CD19, CD20, and CD38 (see ref 18133); continuous monitoring of subpopulations is advised to prevent accidental loss during passaging.
年龄(性别) 29Y
组织来源 腹水
细胞类型 肿瘤细胞
肿瘤类型 淋巴瘤细胞
生物安全等级 BSL-1
保藏机构 DSMZ; ACC-633
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