One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594)

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One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594)-1

中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（红色，Elab Fluor® 594）

货号：GCQ0050

规格：

100 Assays 50 Assays 20 Assays

产品详情

检测原理

细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。

检测方法

荧光法

样本类型

石蜡切片;冰冻切片;细胞爬片;细胞涂片

检测时间

3 hours

检测仪器

荧光显微镜

荧光

Elab Fluor®594

Ex/Em

590/617

Filter set

TRITC

保存温度

该产品可在-20°C避光条件下保存12个月。

有效期

12个月

运输条件

冰袋

产品结果图

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