Mini Sample Mouse VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

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Mini Sample Mouse VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit-1

中文名称：微量法小鼠血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒

货号：GEMSM0005

规格：

96 T 48 T 96 T × 5

产品详情

用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中VEGF-A浓度
产品应用	ELISA
别称	Vegf;Vegfa
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠VEGF-A抗体包被于酶标板上，实验时样品（或标准品）中的小鼠VEGF-A会与包被抗体结合。后依次加入生物素化的抗小鼠VEGF-A抗体和辣根过氧化物酶标记的亲和素，抗小鼠VEGF-A抗体与结合在包被抗体上的小鼠VEGF-A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，VEGF-A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中VEGF-A的浓度。
反应类型	Sandwich-ELISA
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法、ELISA、夹心法
检测范围	7.81-500 pg/mL
灵敏度	3.21 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠VEGF-A，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月

产品结果图

Mini Sample Mouse VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit-产品结果图-1

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