Mini Sample Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

文献(1562) 说明书

一键复制产品信息

Mini Sample Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit-1

中文名称：微量法小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：GEMSM0002

规格：

96 T 48 T 96 T × 5

产品详情

用途

试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度

产品应用

ELISA

检测原理

本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。

反应类型

Sandwich-ELISA

反应时间

3 h 30 min

反应性

Mouse

检测方法

比色法、ELISA、夹心法

检测范围

7.81-500 pg/mL

灵敏度

4.69 pg/mL

样本体积

25 μL

样本类型

血清、血浆或其他生物体液

特异性

可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应

精密度

板内，板间变异系数均<10%

回收率

80%-120%

储存条件

2-8℃

有效期

12个月

产品结果图

相关文献

Anti-inflammatory effect of two novel peptides derived from Binglangjiang buffalo whey protein in lipopolysaccharide-stimulated RAW264.7 macrophages(2023)

期刊：FOOD CHEMISTRY

DOI：10.1016/j.foodchem.2023.136804

影响因子：8.8

相关产品： MS Mouse TNF-α ELISA Kit, MS Mouse IL-6 ELISA Kit
Probiotic-fermented tomato alleviates high-fat diet-induced obesity in mice: Insights from microbiome and metabolomics(2023)

期刊：FOOD CHEMISTRY

DOI：10.1016/j.foodchem.2023.137719

影响因子：8.8

相关产品： MS Mouse TNF-α ELISA Kit, MS Mouse IL-6 ELISA Kit, MS Mouse IL-10 ELISA Kit, Mouse ADP/Acrp30 ELISA Kit, MS Mouse LEP ELISA Kit
Treg-targeted efficient-inducible platform for collagen-induced arthritis treatment(2023)

期刊：Materials Today Bio

DOI：10.1016/j.mtbio.2023.100557

影响因子：8.200

相关产品： MS Mouse IL-2 ELISA Kit, MS Mouse TNF-α ELISA Kit
Bavachin protects against diet-induced hepatic steatosis and obesity in mice(2023)

期刊：ACTA PHARMACOLOGICA SINICA

DOI：10.1038/s41401-023-01056-z

影响因子：8.200

相关产品： MS Mouse TNF-α ELISA Kit
Acid-induced Poria cocos alkali-soluble polysaccharide hydrogel: Gelation behaviour, characteristics, and potential application in drug delivery(2023)

期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

DOI：10.1016/j.ijbiomac.2023.124383

影响因子：8.200

相关产品： MS Mouse TNF-α ELISA Kit, HS Mouse IL-1β ELISA Kit, MS Mouse IL-6 ELISA Kit, MS Mouse IL-10 ELISA Kit, DMEM(High glucose), MEM
Structural characterization and anti-inflammatory activity of polysaccharides from Astragalus membranaceus(2023)

期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

DOI：10.1016/j.ijbiomac.2023.124386

影响因子：8.200

相关产品： MS Mouse IL-6 ELISA Kit, MS Mouse TNF-α ELISA Kit, MS Mouse MCP-1 ELISA Kit
Immuno-stimulatory activity of Astragalus polysaccharides in cyclophosphamide-induced immunosuppressed mice by regulating gut microbiota(2023)

期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

DOI：10.1016/j.ijbiomac.2023.124789

影响因子：8.200

相关产品： QuicKey Pro Mouse IgA ELISA Kit, QuicKey Pro Mouse IgG ELISA Kit, MS Mouse IgM ELISA Kit, HS Mouse IL-1β ELISA Kit, MS Mouse IL-6 ELISA Kit, MS Mouse TNF-α ELISA Kit
Positive interactions among Corynebacterium glutamicum and keystone bacteria producing SCFAs benefited T2D mice to rebuild gut eubiosis(2023)

期刊：FOOD RESEARCH INTERNATIONAL

DOI：10.1016/j.foodres.2023.113163

影响因子：8.1

相关产品： Mouse INS ELISA Kit, MS Mouse TNF-α ELISA Kit, Mouse LBP ELISA Kit, MS Mouse IL-6 ELISA Kit, HS Mouse IL-1β ELISA Kit
Purification and characterization of a glycoprotein from Sipunculus nudus and its immune-enhancing activity to RAW 264.7 macrophages(2023)

期刊：FOOD RESEARCH INTERNATIONAL

DOI：10.1016/j.foodres.2023.113591

影响因子：8.1

相关产品： MS Mouse TNF-α ELISA Kit, HS Mouse IL-1β ELISA Kit, MS Mouse IL-6 ELISA Kit
A Yeast Cell Wall Derived Hybrid Hydrogel with Photothermal and Immune Combined Modality Therapy for Enhanced Anti-Melanoma Efficacy(2023)

期刊：International Journal of Nanomedicine

DOI：10.2147/IJN.S409674

影响因子：8.0

相关产品： MS Mouse TNF-α ELISA Kit, MS Mouse IFN-γ ELISA Kit

产品推荐

Mini Sample Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

联系我们

sales@procell.com.cn

400-999-2100

Mini Sample Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

产品详情

产品结果图

相关文献

产品推荐

联系我们

相关推荐