Mini Sample Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

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Mini Sample Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit-1

中文名称：微量法小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：GEMSM0002

规格：

96 T 48 T 96 T × 5

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用途

试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度

产品应用

ELISA

检测原理

本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。

反应类型

Sandwich-ELISA

反应时间

3 h 30 min

反应性

Mouse

检测方法

比色法、ELISA、夹心法

检测范围

7.81-500 pg/mL

灵敏度

7.81-500 pg/mL

样本体积

25 μL

样本类型

血清、血浆或其他生物体液

特异性

可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应

精密度

板内，板间变异系数均<10%

回收率

80%-120%

储存条件

2-8℃

有效期

12个月

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Mini Sample Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

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