Mini Sample Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：微量法小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
货号：GEMSM0002
价格： ￥3080.00 ￥2180.00 ￥13860.00
产品详情
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度 |
| 产品应用 | ELISA |
| 别称 | DIF;Tnfa;TNF-a;TNFSF2;Tnlg1f;Tnfsf1a;TNFalpha;TNF-alpha;Tnf;Cachectin;Tumor necrosis factor;Tumor necrosis factor ligand superfamily member 2 |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法、ELISA、夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 25 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
产品结果图
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