人脐静脉内皮细胞
英文名称:Human Umbilical Vein Endothelial Cells
货号:GCP-H082
价格: ¥6380.00
产品详情
产品信息
产品名称 | 人脐静脉内皮细胞 |
组织来源 | 脐带组织 |
产品规格 | 5×10^5Cells/T25 |
方法简介 | 普诺赛实验室分离的人脐静脉内皮细胞采用胰蛋白酶-胶原酶联合消化法结合差速贴壁法、并通过内皮细胞专用培养基培养筛选制备而来,细胞总量约为5×10⁵ cells/瓶。 |
质量检测 | 普诺赛实验室分离的人脐静脉内皮细胞经CD31免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。 |
包被条件 | PLL(0.1 mg/mL)或明胶(0.1%) |
培养基 | 人脐静脉内皮细胞完全培养基(工业)(含FBS、生长添加剂、Penicillin、Streptomycin等) () |
换液频率 | 每2-3天换液一次 |
生长特性 | 贴壁 |
细胞形态 | 内皮细胞样 |
传代比例 | 1:2 |
消化液 | Accutase消化液或0.25%胰蛋白酶 |
人脐静脉内皮细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。 |
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